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Comfort This category of cookies is needed to find out how a new website design performs and improve our website. Confirm selection Accept all 1 Introduction 2 Cell types, frequencies, and marker expression 3 Sample preparation Table of Content Spleen 1 Introduction The largest organ of the lymphatic system, the spleen, is responsible for initiating immune reactions to blood-borne antigens and for filtering blood of foreign material and old or damaged blood cells.

Given its role in the lymphatic system, the spleen hosts a broad spectrum of blood cells, both erythrocytes and leukocytes, as well as dead cells, cell debris, and cell fragments (platelets).

At a glance: Kits, reagents, and hardware for the preparation of spleen samples UseCommentsProductKits and reagentsDissociation of spleen tissueEnsures cell surface epitope preservation. Spleen Dissociation Kit, mouseHardware and consumablesTissue dissociationPre-set programs to dissociate different tissues with Miltenyi Biotec enzyme kits for high cell yield and viability.

Migrate to site of infectionDifferentiate into macrophages or dendritic cellsAmong first responders at site of inflammationPathogen destructionRecruitment of immune cellsEnsures cell surface epitope preservation.

Spleen Dissociation Kit, mousePre-set programs to dissociate different tissues with Miltenyi Biotec enzyme kits for high cell yield and viability. Size of the spleen is very variable between individuals, and accessory spleens are very common. The size of spleen varies according to blood flow. Spleen is soft, and pushed against diaphragm by the stomach, left kidney, tail of pancreas, and left flexure of colon.

Spleen is surrounded by a capsule of dense connective tissue, with fibroblasts and collagen covered (reticular fibre) trabeculae extending inwards, dividing the cortex of the spleen into lymphatic nodules (follicles). Venous sinuses contain large volumes of blood, and are surrounded by splenic cords, together called red pulp. Splenic cords are composed of reticular fibres and erythrocytes, macrophages, lymphocytes, plasma cells, and granulocytes.

The size of spleen, mainly red pulp, decreases with ageing. Part of the blood that enters the spleen flows intra-vascularly into the venous sinuses, while another part flows slowly through the lymphatic tissue and extracellular matrix of the red pulp before reaching the venous sinuses.

Rats and humans have sinusoidal circulation, while mice do not. Endothelium consists of longitudinally arranged cells with adjustable slits, enabling cells to squeeze through into the venous sinuses. Erythrocytes that have lost their deformation capacity cannot pass through the slits and are destroyed by sinusoidal phagocytes. Blood from the splenic vein is drained into the portal vein and into the liver.

The spleen does not have afferent lymphatic vessels that lymph nodes have, therefore it collects the white blood cells only from the blood. Lymphatic capillaries and efferent vessels lead to lymph nodes outside of the spleen. In cats and dogs and other carnivores the capsule of the spleen contains significant smooth muscle mass. Rhythmic contractions pump blood plasma to lymphatics, further concentrating the blood cells in the spleen.

In humans the capsule contains only few smooth muscle cells. The spleen is a reticuloendothelial lymphoid organ specialized in filtering blood and producing components of complement and specific antibodies.